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mtnD [2018-10-11 17:55:42]
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mtnD [2018-10-11 17:55:42]

1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Locus
BSU13620
Isoelectric point
4.41
Molecular weight
20.68 kDa
Protein length
178 aa Sequence Blast
Gene length
534 bp Sequence Blast
Function
methionine salvage
Product
1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Essential
no
Synonyms
ykrZ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of methionine/ S-adenosylmethionine

    • Gene

    Coordinates
    1,429,584 → 1,430,120

    The protein

    Catalyzed reaction/ biological activity

  • 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one O2 = 3-(methylthio)propanoate formate CO (according to Swiss-Prot)

    • Protein family

  • acireductone dioxygenase (ARD) family (according to Swiss-Prot)

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    mtnW-mtnX-mtnB-mtnD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • S-box: termination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

    • Regulation

  • induced by methionine starvation (S-box) PubMed
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    Biological materials

    Mutant

  • MGNA-A786 (ykrZ::erm), available at the NBRP B. subtilis, Japan
  • BKE13620 (ΔmtnD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATTCCTCCTTTTA, downstream forward: _UP4_TAAGCGTGAGATAGCCCCGT
  • BKK13620 (ΔmtnD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATTCCTCCTTTTA, downstream forward: _UP4_TAAGCGTGAGATAGCCCCGT

    • References

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